Assessing Post-Traumatic Stress Symptoms in Medical Students of Larkana Using The PCL-5 Scale: A Cross-Sectional Study

Main Article Content

Muhammad Ahsan Qureshi
Gul Bakht Jakhrani
Kainat Soomro
Muhammad Shoaib Panhwar
Muhammad Ayoob Palh
Anoop Kumar Juseja
Mir Hassan Khoso
Nursat Shah
Imran Ali Solangi
Mazhar Malik
Muhammad Ahmed Arain
Nabeela Rajput

Abstract

Background: Post-traumatic stress symptoms may impair psychological well-being, academic functioning, sleep, concentration, and clinical performance among healthcare students, yet local evidence from resource-limited medical education settings in Pakistan remains limited. Objective: To assess the distribution and severity of post-traumatic stress symptoms among healthcare students in Larkana using the DSM-5-aligned PTSD Checklist for DSM-5 and to examine their association with gender, academic department, and age group. Methods: This cross-sectional descriptive-analytical study was conducted from March to May 2025 at a public sector medical university in Larkana, Sindh, Pakistan. A total of 324 students from MBBS, BDS, DPT, Nursing, and Pharmacy programs completed the 20-item PCL-5, rated on a five-point Likert scale. Associations between demographic variables and item-level symptom severity were analyzed using chi-square tests with Monte Carlo exact significance; effect sizes were estimated using Cramér’s V, and post-hoc column proportion tests were applied where appropriate. Results: Complete PCL-5 data were available for all participants. Females showed consistently higher symptom severity across PCL-5 items, with large gender-associated effects (Cramér’s V=0.708–0.720). Departmental differences were moderate, with MBBS students showing greater symptom burden across several domains (Cramér’s V=0.454–0.469). Students aged 18–21 years accounted for the highest proportion of severe intrusion, concentration difficulty, and sleep disturbance, although ordinal age trends were non-significant. Conclusion: Post-traumatic stress symptoms were common and unevenly distributed among healthcare students, with greater symptom concentration among female students, MBBS students, and those aged 18–21 years. Institution-based confidential screening, early counseling, and targeted support for cognitive-emotional and hyperarousal symptoms are warranted.

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1.
Muhammad Ahsan Qureshi, Gul Bakht Jakhrani, Kainat Soomro, Muhammad Shoaib Panhwar, Muhammad Ayoob Palh, Anoop Kumar Juseja, et al. Assessing Post-Traumatic Stress Symptoms in Medical Students of Larkana Using The PCL-5 Scale: A Cross-Sectional Study. JHWCR [Internet]. 2026 May 22 [cited 2026 May 22];4(10):1-14. Available from: https://www.jhwcr.com/index.php/jhwcr/article/view/1640

References

1. Radow B, Anderson N, Richmond BK. Post-traumatic stress disorder in trauma patients. American Surgeon. 2025;91(2):292–299. https://journals.sagepub.com/home/asr

2. Kaplan GB, Lakis GA, Zhoba H. Sleep-wake and arousal dysfunctions in post-traumatic stress disorder. Brain Research Bulletin. 2022;186:106–122. https://doi.org/10.1016/j.brainresbull.2022.05.006

3. Snijders C, de Nijs L, Baker DG, et al. MicroRNAs in post-traumatic stress disorder. Current Topics in Behavioral Neurosciences. 2018;38:23–46. https://link.springer.com/book/10.1007/7854_2017_32

4. Lauten TH, Natour T, Case AJ. Innate and adaptive immune system consequences of post-traumatic stress disorder. Autonomic Neuroscience. 2024;252:103159. https://doi.org/10.1016/j.autneu.2024.10315

5. Yang JJ, Jiang W. Immune biomarkers alterations in post-traumatic stress disorder: systematic review. Journal of Affective Disorders. 2020;268:39–46. https://doi.org/10.1016/j.jad.2020.02.044

6. Leistner C, Menke A. Hypothalamic-pituitary-adrenal axis and stress. Handbook of Clinical Neurology. 2020;175:55–64. https://www.sciencedirect.com/science/article/pii/B9780444641236000047

7. Daskalakis NP, Lehrner A, Yehuda R. Endocrine aspects of post-traumatic stress disorder. Endocrinology and Metabolism Clinics of North America. 2013;42(3):503–513. https://doi.org/10.1016/j.ecl.2013.05.004

8. Goldschen L, Ellrodt J, Amonoo HL, et al. PTSD and systemic lupus erythematosus. Brain, Behavior, and Immunity. 2023;108:292–301. https://doi.org/10.1016/j.bbi.2022.12.012

9. Clayton S. Climate change and mental health. Current Environmental Health Reports. 2021;8:1–6. https://doi.org/10.1007/s40572-020-00303-3

10. Pan X, Kaminga AC, Wu SW, Liu A. Chemokines in post-traumatic stress disorder. Brain, Behavior, and Immunity. 2021;92:115–126. https://doi.org/10.1016/j.bbi.2020.11.033

11. Stopyra MA, Simon JJ, Rheude C, Nikendei C. Neurobiology of PTSD and CPTSD. Reviews in the Neurosciences. 2022;34(1):103–128. https://doi.org/10.1515/revneuro-2022-0014

12. Kirkpatrick HA, Heller GM. PTSD theory and treatment update. International Journal of Psychiatry in Medicine. 2014;47(4):337–346. https://doi.org/10.2190/PM.47.4.h

13. Kube T, Berg M, Kleim B, Herzog P. Predictive processing in PTSD. Neuroscience & Biobehavioral Reviews. 2020;113:448–460. https://doi.org/10.1016/j.neubiorev.2020.04.014

14. Antonelli-Salgado T, Ramos-Lima LF, Machado CDS, et al. Neuroprogression in PTSD. Trends in Psychiatry and Psychotherapy. 2021;43(3):167–176. https://doi.org/10.47626/2237-6089-2020-0099

15. Slavish DC, Briggs M, Fentem A, et al. PTSD and sleep disturbances: systematic review. Sleep Medicine Reviews. 2022;63:101623. https://doi.org/10.1016/j.smrv.2022.101623

16. Messman BA, Fentem A, Compton SE, et al. Affect, sleep and PTSD. Sleep Medicine. 2023;110:287–296. https://doi.org/10.1016/j.sleep.2023.08.025

17. Colvonen PJ, Glassman LH, Crocker LD, et al. Biomarkers predicting PTSD outcomes. Neuroscience & Biobehavioral Reviews. 2017;75:140–156. https://doi.org/10.1016/j.neubiorev.2017.01.027

18. Kanan J, Leão T. PTSD in conflict-exposed youth. Journal of Health Psychology. 2024;29(13):1433–1449. https://doi.org/10.1177/1359105322112314

19. Allen MT. Learning and PTSD vulnerability. Cognitive, Affective & Behavioral Neuroscience. 2023;23(3):869–893. https://doi.org/10.3758/s13415-023-01077-5

20. Cloitre M. ICD-11 complex PTSD. British Journal of Psychiatry. 2020;216(3):129–131. https://doi.org/10.1192/bjp.2020.43

21. Danovitch I. PTSD and substance use disorder. Journal of Addictive Diseases. 2016;35(3):169–179. https://doi.org/10.1080/10550887.2016.1168212

22. Timko CA, Dennis NJ, Mears C, Rodriguez D, Fitzpatrick KK, Peebles R. Post-traumatic stress symptoms in parents of adolescents hospitalized with anorexia nervosa. Eating Disorders. 2023;31(3):212–224. https://doi.org/10.1080/10640266.2022.2099604

23. Hüner B, Friedl T, Schütze S, Polasik A, Janni W, Reister F. Post-traumatic stress syndromes following childbirth influenced by birth mode—is an emergency cesarean section worst? Archives of Gynecology and Obstetrics. 2024;309(6):2439–2446. https://doi.org/10.1007/s00404-023-07114-5

24. Lorem GF, Næss ET, Løchen ML, Lillevoll K, Molund EM, Rösner A, et al. Post-traumatic stress disorder among heart disease patients: a clinical follow-up of individuals with myocardial infarction in the Tromsø Study. BMC Psychiatry. 2023;23:936. https://doi.org/10.1186/s12888-023-05431-2

25. Cankardaş S, Sofuoğlu Z. Post-traumatic stress disorder symptoms and their predictors in earthquake or fire survivors. Turkish Journal of Psychiatry. 2019;30(3):151–156.

26. Li H, Flynn DM, Highland KB, Barr PK, Langford DJ, Doorenbos AZ. Relationship between post-traumatic stress disorder symptoms and chronic pain-related symptom domains among military active duty service members. Pain Medicine. 2021;22(12):2876–2883. https://doi.org/10.1093/pm/pnab087

27. Frommberger U, Angenendt J, Berger M. Post-traumatic stress disorder—a diagnostic and therapeutic challenge. Deutsches Ärzteblatt International. 2014;111(5):59–65. https://doi.org/10.3238/arztebl.2014.0059

28. Yehuda R. Psychoneuroendocrinology of post-traumatic stress disorder. Psychiatric Clinics of North America. 1998;21(2):359–379. https://doi.org/10.1016/S0193-953X(05)70010-1

29. Peters M, Schulz H. Clinical significance of reduced executive functions in mental illnesses. Zeitschrift für Psychosomatische Medizin und Psychotherapie. 2024;70(1):48–62. https://doi.org/10.13109/zptm.2024.70.oa2

30. van der Kolk BA. Developmental trauma disorder: towards a rational diagnosis for chronically traumatized children. Praxis der Kinderpsychologie und Kinderpsychiatrie. 2009;58(8):572–586. https://doi.org/10.13109/prkk.2009.58.8.572

31. Chambers RA, Bremner JD, Moghaddam B, Southwick SM, Charney DS, Krystal JH. Glutamate and post-traumatic stress disorder: toward a psychobiology of dissociation. Seminars in Clinical Neuropsychiatry. 1999;4(4):274–281.

32. Morina N, Kuenburg A, Schnyder U, Bryant RA, Nickerson A, Schick M. The association of post-traumatic and postmigration stress with pain and other somatic symptoms. Pain Medicine. 2018;19(1):50–59. https://doi.org/10.1093/pm/pnx005

33. Charles E, Garand L, Ducrocq F, Clément JP. Post-traumatic stress disorder in the elderly. Psychologie & Neuropsychiatrie du Vieillissement. 2005;3(4):291–300.

34. Fuchs J, Zwemer E, Gillespie A, Zarick P, Berkoff M. Making trauma less traumatic: implementing inpatient pediatric screening of acute stress symptoms. Hospital Pediatrics. 2023;13(8):702–710. https://doi.org/10.1542/hpeds.2022-006930

35. Greene T, Gelkopf M, Grinapol S, Werbeloff N, Carlson E, Lapid L. Trajectories of traumatic stress symptoms during conflict: a latent class growth analysis. Journal of Affective Disorders. 2017;220:24–30. https://doi.org/10.1016/j.jad.2017.05.036

36. Eising CM, Maercker A, Malagurski B, Jäncke L, Mérillat S. Longitudinal resting-state functional connectivity analysis on trauma exposure and PTSD symptoms. NeuroImage: Clinical. 2022;35:103052. https://doi.org/10.1016/j.nicl.2022.103052

37. Shrira A, Ayalon L, Bensimon M, Bodner E, Rosenbloom T, Yadid G. Parental PTSD symptoms and successful aging in offspring of Holocaust survivors. Frontiers in Psychology. 2017;8:1099. https://doi.org/10.3389/fpsyg.2017.01099

38. Levin Y, Mazza A, Hyland P, Karatzias T, Shevlin M, McGinty G, et al. Positive symptoms of psychosis and complex PTSD: a network analysis. Canadian Journal of Psychiatry. 2025;70(7):583–592. https://doi.org/10.1177/07067437251337645

39. Bartels L, Skar AS, Birkeland MS, Ormhaug SM, Berliner L, Jensen TK. PTSD symptoms and functional impairment in children and adolescents. European Child & Adolescent Psychiatry. 2024;33(5):1573–1581. https://doi.org/10.1007/s00787-023-02266-w

40. Neugebauer R, Fisher PW, Turner JB, Yamabe S, Sarsfield JA, Stehling-Ariza T. PTSD reactions in Rwandan youth after genocide. International Journal of Epidemiology. 2009;38(4):1033–1045. https://doi.org/10.1093/ije/dyn375

41. Knefel M, Karatzias T, Ben-Ezra M, Cloitre M, Lueger-Schuster B, Maercker A. Complex PTSD symptom networks in adults. The British Journal of Psychiatry. 2019;214(6):361–368. https://doi.org/10.1192/bjp.2018.286

42. Dimitrova L, Fernando V, Vissia EM, et al. Trauma and dissociation in PTSD. European Journal of Psychotraumatology. 2020;11(1):1705599. https://doi.org/10.1080/20008198.2019.1705599

43. van Veldhoven LM, Sander AM, Struchen MA, et al. Stressful life events and PTSD after mild traumatic brain injury. Journal of Neurology, Neurosurgery & Psychiatry. 2011;82(7):782–787. https://doi.org/10.1136/jnnp.2010.228254

44. Zhang H, Hu Y, Yu Y, et al. Neuroimaging in PTSD. Translational Psychiatry. 2025;15:208. https://doi.org/10.1038/s41398-025-03416-1

45. Peltzer K. PTSD in university students. Central African Journal of Medicine. 1998;44(11):280–283.

46. Nakell L. Adult PTSD: screening and treatment in primary care. Primary Care. 2007;34(3):593–vii. https://doi.org/10.1016/j.pop.2007.05.010

47. Dyball D, Maqsood R, Schofield S, et al. PTSD symptom clusters and heart rate variability. Journal of Affective Disorders. 2025;375:86–92. https://doi.org/10.1016/j.jad.2025.01.087

48. Hassan AN, Le Foll B, Imtiaz S, Rehm J. PTSD and risk of opioid use disorder. Drug and Alcohol Dependence. 2017;179:260–266. https://doi.org/10.1016/j.drugalcdep.2017.07.012

49. Herbst G, Jaeger U, Leichsenring F, Streeck-Fischer A. Effects of trauma in adolescents. Praxis der Kinderpsychologie und Kinderpsychiatrie. 2009;58(8):610–634.

50. Maujean A, Gullo MJ, Andersen TE, Ravn SL, Sterling M. PTSD symptom clusters in whiplash disorder. Pain Reports. 2017;2(6):e631. https://doi.org/10.1097/PR9.0000000000000631

51. Xu G, Li G, Yang Q, Li C, Liu C. Repetitive transcranial magnetic stimulation in PTSD: meta-analysis. Stress and Health. 2024;40(1):e3292. https://doi.org/10.1002/smi.3292