Antibiotic Resistance Pattern of E. Coli Isolated from UTI Patients at Mayo Hospital
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Background: Urinary tract infection is one of the most common bacterial infections encountered in clinical practice, and Escherichia coli remains the leading uropathogen. Increasing antimicrobial resistance among urinary E. coli isolates has reduced the effectiveness of commonly used empirical antibiotics and highlights the need for local susceptibility surveillance. Objective: To determine the antibiotic resistance pattern of Escherichia coli isolated from urinary tract infection patients at Mayo Hospital, Lahore. Methods: A descriptive cross-sectional study was conducted over six months in the Clinical Microbiology Laboratory of Mayo Hospital, Lahore. A total of 200 culture-positive urine samples from patients with urinary tract infection were processed using standard microbiological methods. Bacterial isolates were identified by colony morphology, Gram staining, biochemical testing, and VITEK-2 Compact system. Antimicrobial susceptibility testing of confirmed E. coli isolates was performed using the Kirby–Bauer disk diffusion method according to Clinical and Laboratory Standards Institute guidelines. Results: E. coli was the predominant uropathogen, isolated from 142 of 200 samples (71.0%). Among E. coli cases, 96 (67.6%) were female and 46 (32.4%) were male. The highest sensitivity was observed for imipenem 140/142 (98.6%), fosfomycin 126/142 (88.7%), and nitrofurantoin 120/142 (84.5%). The highest resistance was observed against nalidixic acid 115/142 (81.0%), Septran 98/142 (69.0%), and cefoperazone 78/142 (54.9%). Conclusion: E. coli was the major uropathogen among urinary tract infection patients, with substantial resistance to commonly used antibiotics. Routine urine culture, antimicrobial susceptibility testing, rational antibiotic prescribing, and continuous local resistance surveillance are essential to guide effective treatment.
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